"Shaping Light for Bio-Imaging"

Jung-Hoon Park

(Ulsan National Institute of Science and Technology (UNIST))

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Date: May 31, 2023


As we can only see the intensity of light, it is not easy to see the importance of the phase of light in our everyday lives. Here, we will describe our recent developments in controlling the wavefront, or in other words, the phase of light to realize advanced optical systems. First, we demonstrate that controlling the wavefront of light can enable high resolution imaging inside aberrating deep tissue and realize functional imaging of brain dynamics in live mice. We also demonstrate that hardware based wavefront shaping is not the only solution and describe a computational approach to image through dynamic turbid media based on the principles of speckle interferometry. In a different perspective, we demonstrate how we can shape the illumination beam to enable different imaging characteristics for different regions of interests within the FOV simultaneously. For example, by illuminating selective sinusoidal illumination patterns, we realize tunable SIM, a novel dynamic adaptive structured illumination microscope, which realizes varying spatiotemporal resolutions across the FOV that can adapt to the user’s needs. Tunable SIM is realized using a DMD and the simplicity of the setup will be ideal for usability and broad dissemination. We further discuss how we can use custom illumination to enable stroboscopic, high dynamic range, or selective depth excitation imaging for advanced bio imaging applications.

Further Information:

Jung-Hoon is an Associate Professor at the Department of Biomedical Engineering at UNIST. He received his Ph.D in Physics at KAIST and conducted postdoctoral research studies at Janelia Research Campus. His research interests focus on building novel optical systems and computational methods to enable high resolution, high speed deep tissue imaging, especially for the brain.



Created: Thursday, June 1st, 2023