Compressive light-field microscopy for 3D functional imaging of the living brain

Nicolas Pegard

Nicolas Pégard

(UC Berkeley)

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Date: April 7, 2016


We present a new microscopy technique for 3D functional neuroimaging in live brain tissue. The device is a simple light field fluorescence microscope allowing full volume acquisition in a single shot and can be miniaturized into a portable implant. Our computational methods first rely on spatial and temporal sparsity of fluorescence signals to identify and precisely localize neurons. We compute for each neuron a unique pattern, the light-field signature, that accounts for the effects of optical scattering and aberrations. The technique then yields a precise localization of active neurons and enables quantitative measurement of fluorescence with individual neuron spatial resolution and at high speeds, all without ever reconstructing a volume image. Experimental results are shown on live Zebrafish.

Further Information:

Nicolas Pégard received his B.S. in Physics from Ecole Polytechnique (France) in 2009, and his Ph.D. in Electrical Engineering at Princeton University under Prof. Fleischer in 2014. He is now a postdoctoral researcher at U.C. Berkeley under the supervision of Prof. H.Adesnik (Molecular and Cell Biology dpt.) and Prof. L.Waller. (Electrical Engineering and Computer Science dpt.) His main research interests are in optical system design and computational microscopy. He is currently developing all-optical methods to observe and control the activity of individual neurons in deep, live brain tissue with high spatial and temporal resolution.

Created: Thursday, April 7th, 2016