Multi-Spectral Optoacoustic Tomography – Volumetric Color Hearing in Real-time


Daniel Razansky

(Technical University Munich)

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Date: 04/05/2012


The main‐stream of optical interrogations in living tissues are still limited to surface‐limited microscopic imaging or otherwise low resolution diffusion tomographies. Despite significant progress in both fields, those methods do not allow exploration of the full potential of novel classes of fluorescent and other molecular agents for high‐resolution volumetric quantitative imaging of entire organs, small animals or human tissues. Biomedical optoacoustics has emerged in the recent decade as a powerful tool for high‐resolution visualization of optical contrast, overcoming a variety of longstanding limitations imposed by light scattering in deep tissues. But true performance of optoacoustic imaging techniques can only be exploited when excitation at multiple wavelengths is used in order to enable highly sensitive spectral differentiation of intrinsic biomarkers and extrinsically administered contrast agents. By detecting tiny sound vibrations, resulting from selective absorption of light at multiple wavelengths, multispectral optoacoustic tomography (MSOT) can now “hear color” in three dimensions, i.e., deliver volumetric spectrally enriched (color) images from deep living tissues at high spatial resolution and in real time. These new‐found imaging abilities directly relate to preclinical screening applications in animal models and are foreseen to significantly impact clinical decision making as well. The talk provides the technical essentials of MSOT, including latest developments in the inverse theory,spectral processing algorithms, and imaging instrumentation. Several in‐vivo imaging studies, involving gene expression and other molecular agents, are showcased in small animals with performance that forecasts MSOT as a method of choice for biological imaging and select clinical segments.

Created: Thursday, April 5th, 2012