Quantitative single molecule analysis of conformational dynamics in a GPCR signaling system

Reza Vafabakhsh

(University of California, Berkeley)

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Date: February 9, 2016


Metabotropic glutamate receptors (mGluRs) are members of the group C family of GPCRs and are responsible for the regulation of neuronal excitability and synaptic transmission across the CNS. In this seminar, I will present our work on developing quantitative methods to probe the conformation of mammalian mGluRs at the single protein level and I will describe a general model for the activation of mGluRs. I will then discuss the quantitative analysis of the complexity of the mGluR activation process among structurally similar subtypes. Next I will present our results on quantifying the conformational dynamics of a potassium channel, a model system for downstream effector of the GPCR signaling. Finally, I will discuss how this experimental strategy should be widely applicable to quantitatively study conformational dynamics in GPCRs and other membrane protein complexes.

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This talk is available to Stanford faculty, staff, and students.

Created: Tuesday, February 16th, 2016