Optics in Pathology: from Superresolution Microscopy to Point of Care Devices


Sebastian Wachsmann Hogiu

(University of California, Davis)

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Date: 04/05/2012


In this talk I will present our group’s work to develop and apply novel optical technologies for pathology. One such technology is aimed at improving spatial resolution in optical microscopy. This technique (called super‐resolution optical microscopy) utilize either patterned illumination or photo‐physical processes in molecules, and can, in certain situations, resolve objects as small as tens of nanometers. We will make use of a super‐resolution microscope that is based on structured illumination for analysis of blood and other types of cells on pathology slides. The spatial resolution that can be achieved with this microscope is approximately 100nm, and allows for the morphological investigation of fluorescent structures within cells. Another example is the development of two attachments to a commercial cell phone that transform the phone’s integrated lens and image sensor into a 350x microscope and visible‐light spectrometer. The microscope is capable of transmission and polarized microscopy modes and is shown to have 1.5 micron resolution and a usable field‐of‐view of ~150×150 microns with no image processing, and approximately 350×350 microns when post‐processing is applied. The spectrometer has a 300 nm bandwidth with a limiting spectral resolution of close to 5nm. We show applications of the devices to medically relevant problems. In the case of the microscope, we image both stained and unstained blood‐smears showing the ability to acquire images of similar quality to commercial microscope platforms, thus allowing diagnosis of clinical pathologies.

Created: Thursday, April 5th, 2012